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Two distinct, sequence-specific DNA-binding proteins ...

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.

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Role of Asymmetric Dimethylarginine(ADMA)in ... - …

to a SDS Tris -Glycine gradient gel 4-12% (Invitrogen) and run at 130V for 2 hours. The gel was then removed and the protein was transferred on to …

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Jun 02, 2005· Various embodiments of the invention provide human enzymes (ENZM) and polynucleotides which identify and encode-ENZM. Embodiments of the invention also provide expression vectors, host cells, antibodies, agonists, and antagonists.

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Summary Data from 1668 men (316 cardiovascular disease events) from the Framingham Offspring Study was reanalysed, specifically examining APOE: smoking interactions. Overall hazard ratio (HR) for smoking was 1.95 (1.52, 2.50) compared to non-smokers.

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(PDF) Protection of vascular cells from oxidative stress ...

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Impaired Nitric Oxide Synthase Pathway in Diabetes ...

Background— An endogenous inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA), is elevated in patients with type 2 diabetes mellitus (DM). This study explored the mechanisms by which ADMA becomes elevated in DM. Methods and Results— Male Sprague-Dawley rats were fed normal chow or high-fat diet (n=5 in …

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Interestingly, the same group later found that by omitting the “boiling step” prior to SDS-PAGE, a novel colabeled 38 kDa protein was revealed . The Bryan laboratory demonstrated the 38 kDa protein to be Kir6.2, which provided the first biochemical evidence for …

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Baseline characteristics of the 2 study groups were described by means and SDs, medians and interquartile ranges (IQRs), or percentages, as appropriate, for the level of measurement and distributions of the data.

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